2. TEST FOR REDUCING AND NON-REDUCING SUGARS
(a) Reducing sugar can reduce copper II ion (퐶푢2+) which is blue to copper (I) ion (퐶푢+)
which is orange or brick red. During reduction process, the colour of Benedict’s
solution changes from blue, to green, to yellow and finally to orange or brick red
depending on the concentration of reducing sugar. If it is high, the colour changes
reaches to brick red but if it is low it can end up to the intermediate colours that
have been mentioned. Examples of reducing sugar; glucose, fructose, galactose,
maltose and lactose. Food sources of reducing sugar are onions and carrots.
(b) Non-reducing sugar such as sucrose cannot reduce copper II ions (퐶푢2+) to copper
(I) ions (퐶푢+). Therefore, it cannot be tested directly by Benedict’s solution.
▪
It is first converted to reducing sugar by using hydrochloric acid (HCl) in a
process called hydrolysis. The acid mixture is heated and left to cool.
HCl
C12H22O11 + H2O
C6H12O6 + C6H12O6
Glucose + Fructose
Sucrose
+ Water
▪
The acidic mixture is the neutralized by using sodium hydroxide (NaOH) or
sodium bicarbonate (푁푎퐻퐶푂3) for the Benedict’s solution to work properly.
Food sources containing non-reducing sugar is sugarcane.
Food
Procedures
Observation
Inference
Tested
▪
▪
Place 2mls of a food sample solution
into a clean and dry test tube.
The
colour Reducing
changed from blue sugar
Reducing
sugar
Then,
add an equal
volume of to green, yellow, present
orange and finally
to brick-red.
Benedict’s solution and heat gently
to boil.
▪
▪
Place 2mls of a food sample into a The
colour Non-
clean and dry test tube.
Then, add 2mls
changed from blue reducing
of
dilute to green, yellow, Sugar
hydrochloric acid to the solution orange and finally present
and boil for 3 minutes to hydrolyze to brick-red.
the sugar.
Non-
reducing
Sugar
▪
▪
Leave the solution to cool, and then
add 2mls of sodium hydroxide
solution
or
sodium
hydrogen
carbonate to neutralize the acid.
Add 2mls of Benedict’s solution,
then heat again to boil.